Anti Glycoprotein-340 (gp-340)
Mouse monoclonal antibody
Cat.No. HYB 213-01
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Glycoprotein-340 is member of the scavenger receptor superfamily and has been identified as a putative SP-D receptor. Gp-340 has been shown to bind SP-D in a calcium dependent manner through a protein-protein interaction. Like SP-D, gp-340 is mainly found in lung tissue and immunohistochemistry revealed high expression in alveolar macrophages (1). An identical protein is present in saliva (salivary agglutinin) and both forms are encoded by the gene DMBT1 (5).
Native gp-340 purified from bronchioaveolar lavage from proteinosis patients
HYB 213-01 is specific for gp-340, but reacts with a carbohydrate epitope and cross-reacts with saliva MUC7 in immunohistochemistry.
Not determined
In Western blotting after SDS-PAGE HYB 213-01 reacts strongly with gp-340 in both reduced and non-reduced forms. Strong reaction is seen in ELISA with gp340 directly coated onto the microtiter well.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:60000 | |
| Immunoblotting | Yes | 1:1000 | 2 |
| Immunohistochemistry | Yes | 1:50 | 1, 2, 3, 4 |
1. Holmskov U, Mollenhauer J, Madsen J, Vitved L, Gronlund J, Tornoe I, Kliem A, Reid KB, Poustka A, Skjodt K (1999) Cloning of gp-340, a putative opsonin receptor for lung surfacant protein D. Proc Natl Acad Sci USA 96:10794-10799.
2. Holmskov U, Lawson P, Teisner B, Tornøe I, Willis AC, Morgan C, Koch C, Reid KBM (1997) Isolation and characterization of a new member of the scavenger receptor superfamily, Glycoprotein-340 (gp-340), as a lung surfactant protein-D binding molecule. J Biol Chem 272:13743-13749.
3. Bikker FJ, Ligtenberg AJ, van der Wal JE, van der Keijbus PA, Holmskov U, Veerman EC, Nieuw Amerongen AV (2002) Immunohistochemical detection of salvary agglutinin/gp-340 in human parotid, submandibular, and labial salivary glands. J Dent Res 81:134-139.
4. Ligtenberg TJ, Bikker FJ, Groenink J, Tornoe I, Leth-Larsen R, Veerman EC, Nieuw Amerongen AV, Holmskov U (2001) Human salivary agglutinin binds to lung surfactant protein-D and is identical with scavenger receptor protein gp-340. Biochem J 359:243-248.
5. Bikker FJ, Ligtenberg AJ, Nazmi K, Veerman EC, van't Hof W, Bolscher JG, Poustka A, Nieuw Amerongen AV, Mollenhauer J (2002) Identification of the bacteria-binding peptide domain on salivary agglutinin (gp-340/DMBT1), a member of the scavenger receptor cysteine-rich superfamily. J Biol Chem 277:32109-32115.
Larger Picture (419kB)
Localization of gp-340 by immunohistochemical staining of paraffin embedded section of human lung with a streptavidin biotin immunocytochemical technique. The immunoreaction product, stained with HYB 213-01, is red and the section was counterstained with Mayers hematoxylin.
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April 23 2008
